隐源性食酸丛毛单胞菌血流感染调查研究 FREE

Cryptogenic bloodstream infection with Comamonas acidovorans FREE

摘要:

目的调查某医院临床科室分离的8株食酸丛毛单胞菌的同源性，探讨其导致医院血流感染的途径。方法对分离的食酸丛毛单胞菌，采用随机引物扩增多态性DNA技术检测并进行同源性分析，同时采集病房环境和医疗用品样本进行培养。结果8株食酸丛毛单胞菌为同一基因型，而病房环境和医疗用品中未分离出此菌。结论此起食酸丛毛单胞菌血流感染为集中发病，病原菌为同一基因型，但感染源未明。  

Abstract:

ObjectiveTo analyze the homology of 8 strains of Comamonas acidovorans (C. acidovorans ) isolated from a clinical department of a hospital, and evaluate the routes of bloodstream infections caused by C. acidovorans.MethodsEight strains of C. acidovorans were isolated from samples collected from patients and medical devices. C. acidovorans were identified with biochemical analysis, and their homology were determined with randomly amplified polymorphic DNA (RAPD) .ResultsEight strains of C. acidovorans had the same genotype, no C. acidovorans was isolated from hospital environment and medical devices.ConclusionBloodstream infection with C. acidovorans in hospital is concentrative and cryptogenic, pathogens were of the same genotype.