Abstract:The HACEK group microorganisms (Haemophilus parainfluenzae, Aggregatibacter actinomycetemcomitans, Cardiobacterium spp., Eikenella corrodens, Kingella kingae) belong to Gram-negative bacilli and are members of the normal oropharyngeal microbiota, which can cause invasive opportunistic infection when the host immune barrier is damaged, with infective endocarditis being one of the most serious diseases. HACEK endocarditis is characterized by an insidious course, prolonged delay of diagnosis, and dependence on third-generation cephalosporins for treatment. In recent years, with the emergence and development of 16S ribosomal RNA gene sequencing technique, the rapid diagnosis of this disease is expected to be achieved. This paper summarizes the main clinical characteristics and research progress of infective endocarditis caused by HACEK group bacteria, aiming to provide reference for clinical diagnosis and treatment.