Abstract:Objective To observe the expression of pro-inflammatory factors including interleukin (IL)-1α, IL-1β, IL-6 and IL-8 after silencing and over-expressing of tumor necrosis factor receptor-associated factor 6 (TRAF6) in vitro, explore the effect of TRAF6 on pro-inflammatory factors after Kaposi’s sarcoma-associated herpesvirus (KSHV) infection, and provide new ideas for the study of the pathogenesis of KSHV. Methods The silence and over-expression TRAF6 models were established using KSHV(+)iSLK cell line, and the transfection level was detected by real-time fluorescence quantitative polymerase chain reaction (RT-PCR). After 48 and 72 hours of cultivation of cells, the expression levels of pro-inflammatory factors after silencing and over-expressing TRAF6 were detected by enzyme-linked immunosorbent assay (ELISA) and compared. Results TRAF6-silence (siTRAF6) and TRAF6-over-expression (TRAF6-OE) cell models were established successfully. ELISA results showed that pro-inflammatory factors IL-1α, IL-1β, IL-6, and IL-8 increased in varying degrees after silencing TRAF6, while these pro-inflammatory factors decreased in varying degrees after TRAF6 over-expression, IL-6 and IL-8 levels changed remarkablely, suggesting that TRAF6 had a certain inhibitory effect on pro-inflammatory factors and played an important role in the signaling pathway of pro-inflammatory effects after KSHV infection. Conclusion TRAF6 has a certain inhibitory effect on pro-inflammatory factors and is expected to serve as a potential target to inhibit the progression of Kaposi’s sarcoma.