Abstract:Objective To reveal characteristics of molecular typing of clinically isolated Enterococcus faecalis (E. faecalis) and its relationship with susceptibility to telithromycin. Methods 320 strains of E. faecalis isolated from different clinical specimens in Nanshan Hospital of Shenzhen City from 2010 to 2016 were collected, genotyping of E. faecalis was performed by multilocus sequence typing (MLST), minimum inhibitory concentration of telithromycin to strains was determined by broth microdilution method, and distribution of drug-resistant gene ermB was detected by polymerase chain reaction (PCR). Results 320 strains of E. faecalis were divided into 48 ST types and 56 new ST types, mainly ST16 and ST179. 85 strains (26.6%) were ST16, 72 (84.7%) of which were positive for ermB, 29 (34.1%) were susceptible to telithromycin, 10 (11.8%) were intermediate, 46(54.1%) were resistant to telithromycin; 80 strains (25.0%) were ST179, 71(88.8%) of which were positive for ermB, 15(18.8%) were susceptible to telithromycin, 4 (5.0%) were intermediate, and 61 (76.3%) were resistant to telithromycin. Conclusion ST16 and ST179 are dominant strains of E. faecalis in this hospital, they carry ermB gene in a high proportion and have high resistance rate to telithromycin.