Abstract:Objective To detect status and scope of faucet contamination in intensive care unit(ICU), so as to provide basis for rational layout around the faucets and reducing water-borne infection. Methods Cotton-swab sampling method was used to take specimens from two faucet outlets and their surroundings in ICU of a tertiary general hospital, taking the outlet as the center, each specimen was taken every 20-30 cm, specimens were detected by ATP technique, conventional microbial detection and next-generation sequencing technique(NGS), contamination status and splash range around faucets and their surroundings were analyzed. Results In the case of conventional cleaning, faucets and their surrounding area within 70 cm was highly contaminated. The qualified rates of ATP detection and conventional microbial detection were 60.0% and 73.3% respectively. ATP detection results of two faucet outlets were 2 526 RLU/piece and 687 RLU/piece respectively, microbial detection showed that the number of colonies were both>105 CFU/cm2. In the area 70 cm away from the outlets, the number of bacterial colonies still exceeded the standard. The NGS detection showed that Moraxella osloensis with high coverage was found from the faucet outlets to their surroundings, which was consistent with the distribution of bacteria at the outlets. Conclusion Faucet is an important source of contamination in the ward, cleaning of faucet and its surroundings should be paid attention, clean articles and articles related to patients should not be placed within 1 meter.