Abstract:ObjectiveTo investigate carbapenemase genes and molecular epidemiological characteristics of carbapenemresistant Klebsiella pneumoniae(CRKP), so as to provide laboratory basis for healthcareassociated infection（HAI） control and clinical treatment. Methods13 strains of CRKP isolated from Taian City Central Hospital between January and November 2014 were collected, bacterial identification and antimicrobial susceptibility testing were performed by WalkAway 96 PLUS automatic bacterial analyzer; carbapenemase phenotypes were confirmed by modified Hodge test and EDTAdisk synergy test; carbapenem resistance genes (blaKPC, blaIMP, blaVIM, blaGIM, and blaNDM1) were amplified by polymerase chain reaction (PCR), then sequenced; clone correlation of strains was investigated by pulsedfield gel electrophoresis (PFGE) and multilocus sequence typing (MLST), epidemiology comparison was performed. Results13 strains of CRKP were mainly isolated from sputum (n=7, 53.85%) and urine (n=4, 30.77%), resistance rates to compound sulfamethoxazole and tetracycline were all low (all <40%), to the other antimicrobial agents (except amikacin) were all>70%, resistance rates to carbapenems were all 100%; 13 strains were all positive for modified Hodge test, 5 strains were positive for EDTAdisk synergy test; sequencing of PCR confirmed that blaKPC gene was most common among carbapenemase genes (13/13), followed by blaNDM1 gene (5/13), while blaIMP, blaVIM, and blaGIM gene were not found; clustering analysis of PFGE showed that 13 strains of Klebsiella pneumoniae were divided into 5 types, mainly type C (9/13), all belonged to ST11, others were ST37, ST626, ST628, and ST668 respectively. ConclusionCarbapenemase genes blaKPC and blaNDM1 are the main causes of the resistance of Klebsiella pneumoniae to carbapenems in this hospital, ST11 is the main clone type, hospital should strengthen the prevention and control of HAI as soon as possible.