Homology analysis on human and animalderived Escherichia coli

Abstract:

ObjectiveTo study the homology of Escherichia coli(E. coli) isolated from a large scale farm and patients in a hospital. MethodsFour strains of polymyxinresistant E. coli were isolated from a large scale farm in Tai’an, 12 strains of carbapenemresistant E. coli were isolated from patients in Tai’an Central Hospital; target strains were performed resistance gene sequencing, homology detection was performed by multilocus sequence typing (MLST) and pulsedfield gel electrophoresis (PFGE), homology between E. coli from human and duck were analyzed. ResultsThere were 16 strains of E. coli, 2 of 4 duckderived strains were ST5912; 5 of 12 humanderived strains were ST167, 3 were ST405; 1 duckderived strain and 1 humanderived strain was of the same ST, both were ST10, but PFGE showed that their similarity was 64.7%. ConclusionHomology between humanderived and duckderived E. coli strains in Tai’an area is not very high.