Detection of drug resistance genes and homology of carbapenemresistant Klebsiella pneumoniae

Abstract:

ObjectiveTo explore the drugresistant genotypes and homology of carbapenemresistant Klebsiella pneumoniae (CRKP). Methods38 strains of clinically isolated CRKB in a hospital between September 2015 and February 2016 were collected, drug resistance genes were detected with polymerase chain reaction (PCR), homology of strains was analyzed with pulsedfield gel electrophoresis (PFGE). ResultsAll 38 strains were from intensive care unit(ICU) and surgical intensive care unit(SICU), accounting for 39.48% and 34.21% respectively. 38 strains all harboured drug resistance genes blaKPC and blaSHV , 6 strains harboured blaCTX. PFGE revealed that strains were divided into types A, B, C, and D, type C was predominant（65.78%，25/38）. Of type A strains, strains 14, 15, and 16 carried drug resistance genes blaKPC2, blaSHV, and blaCTXM15 respectively, these strains were all isolated from SICU patients, strains 14 and 15 were isolated on the same day, strain 16 was isolated on the following week; of type C strains, homology of strain 10, 18, 25, and 28 was 100%. strian 10 and 18 were isolated from ICU patients, strains 25 and 28 were isolated from patients in division I of neurology ICU( both were transferred from ICU), and both were isolated during patient hospitalization in ICU, the isolation time only differed for one day.ConclusionThe main drugresistant genotypes of CRKB in this hospital are mainly blaKPC and blaSHV,  there is epidemic of clone strains in this hospital.