Abstract:ObjectiveTo understand the prevalence and risk factors of healthcareassociated infection(HAI), and provide evidence for prevention and control of HAI. MethodsA crosssectional survey was adopted, bedside survey and medical record reviewing method was combined to investigate and analyze the prevalence of HAI in a tertiary firstclass hospital in 2012-2015. ResultsA total of 4 725 hospitalized patients were surveyed,the prevalence rates in 2012-2015 were 6.00%,4.77%,3.93%,and 3.05% respectively, difference was significant(P<0.05); antimicrobial usage rates were 30.56%,33.82%,32.84%, and 34.48% respectively, difference was not significant(P>0.05);the main infection site was lower respiratory tract(43.00%),followed by surgical site (16.43%); the risk factors for HAI were age ≥65 years, chronic systemic diseases(diabetes, cirrhosis, chronic renal failure, chronic lung disease), immunodeficiency(white blood cell<1.5×109/L), coma, tracheotomy, and mechanical ventilation. ConclusionSurvey on HAI prevalence can promote continuous improvement of HAI management, surveillance on surgical site infection and risk factors of HAI should be strengthened.