Expression and variation of MIP-1β, MIP-2, and IL-12p70 in mouse models with bloodstream infection caused by different bacteria

Abstract:

ObjectiveTo investigate the expression and variation of MIP1β, MIP2, and IL12p70 in mice with bloodstream infection caused by 4 kinds of bacteria.MethodsCD1 (ICR) mouse models of bloodstream infection with Staphylococcus aureus (S. aureus), Enterococcus faecalis(E. faecalis), Escherichia coli(E. coli), and Klebsiella pneumoniae(K. pneumoniae) were established. After mice in each trial group and PBS control group were infected by bacteria for 0.5h, 1h, 3h, 6h, 12h, 24h, and 48h, concentrations of MIP1β, MIP2, and IL12p70 were detected by Luminex liquid suspension chip system.ResultsConcentrations of MIP1β increased significantly 1h after bacteria was in blood, S. aureus, E. faecalis, E. coli, K. pneumoniae, and control groups were (134.5±18.3), (61.5±15.4), (3 354.0±809.0), (6 888.4±1 100.2), and (28.9±4.6) pg/mL respectively; the peak values of IL12p70 were (389.3±118.1), (127.6±10.0), (42.2±3.5), (62.8±8.4), and (4.8±0.3) pg/mL respectively. Concentrations of MIP1β and MIP2 in E. coli and K. pneumoniae groups were significantly higher than other trial groups and control group (all P<0.01), while concentrations of IL12p70 in S. aureus and E. faecalis groups were both significantly higher than E. coli, K. pneumoniae, and control groups (all P<0.01).ConclusionConcentrations of MIP1β and MIP2 in E. coli and K. pneumoniae groups were both significantly higher than those in S. aureus and E. faecalis groups, while concentrations of IL12p70 in S. aureus and E. faecalis groups were both significantly higher than those in E. coli and K. pneumoniae groups. The combination detection of multiple cytokines or chemokines are valuable in predicting grampositive or gramnegative bacterial infection, and can provide basis for treatment of early infection.