Abstract:ObjectiveTo investigate the hygienic status of large bottles of medical ultrasonic coupling gel in medical institution. MethodsFrom February 2012 to April 2016, microbial contamination of large bottles of medical ultrasonic coupling gel in a women and children’s hospital was investigated and analyzed through onthespot random sampling and detection method. ResultsA total of 170 large bottles of medical ultrasonic coupling gel specimens were collected, 25 specimens were qualified, the qualified rate was 14.71%. Specimens were mainly from inpatient wards(58.24%) and operating departments(21.76%); there was no statistical difference in the qualified rate of specimens in each department(P>0.05). Contamination rates of coupling gel before and after the opening were both>80%, difference was not significant(P>0.05). A total of 145 strains of pathogenic bacteria were isolated, 18 of which were from unopened bottles, and 127 from opened bottles. Burkholderia cepacia was the main strain in both unopened and opened bottles, which accounting for 83.33% and 54.33% respectively，in addition, Pseudomonas aeruginosa and Serratia marcescens were also isolated from opened bottles, both were 15.75%, mixed contamination bacteria all included Serratia marcescens. ConclusionThe total bacteria counts in medical ultrasonic coupling gel in large bottles exceed the standard seriously, the manufacturer should strictly observe the quality control standards, medical institutions should adopt effective cleaning and disinfection measures.