Quantitative detection on different HBsAg levels by chemiluminescence immunoassay and timeresolved immunofluorescence assay

Abstract:

ObjectiveTo evaluate the accuracy and feasibility of timeresolved immunofluorometric assay (TRIFA) for detection of HBsAg based on Abbott automated chemiluminescence immunoassay(CMIA), so as to carry out this project in primary hospitals, and provide reference for individual antiviral strategy and prediction of therapeutic effect. MethodsSerum of 157 patients infected with hepatitis B virus were detected with CMIA and TRIFA, specimens with HBsAg titers exceeding the detection limit were firstly diluted, then performed quantitative analysis. HBsAg levels were divided into 4 groups: ≤100 IU/mL, 101-1 000 IU/mL, 1 001-20 000 IU/mL, and ＞20 000 IU/mL， quantitative correlation between two methods was analyzed. ResultsThe linear regression equation of two methods was Y=2.323X-896.3, correlation coefficent r=0.943, P<0.001. CMIA was as a reference, 4 groups were divided for analysis, results showed that when detected specimens was at low concentration of HBsAg, TRIFA value was low compared with CMIA method, while detected specimens was at high concentration of HBsAg, CMIA value was high, two reagents had good consistency in the detection of different concentrations of HBsAg(both P<0.05), when concentration was at 1 001-20 000 IU/mL, consistency was the best. ConclusionThe accuracy of two reagents in the quantitative detection of HBsAg is similar, and the best correlation of detection value is 1 000-20 000 IU/mL. TRIFA assay has wide application for its lowcost and easy to be operated, which is especially suitable for primary hospitals.