Abstract:ObjectiveTo investigate cognitive status of medical students of a medical university on laboratory biosafety, and provide basic data for laboratory biosafety management in Chinese universities.Methods900 fulltime undergraduate medical students were chosen by cluster random sampling, questionnaires were filled out in by them.Results900 questionnaires were distributed, 877 (98.21%) valid questionnaires were obtained, 49.03% (n=430) were from sophomores, 50.97%（n=447）from juniors，148（16.88%）students have ever participated in students’ scientific research. The overall awareness rate of laboratory biosafety was 58.72%, only 32.16% of students understood the detailed contents of laboratory biosafety regulations, only 8.21% of students have received training in laboratory biosafety; the awareness rate of laboratory biosafety cabinet was only 14.14%, only 7.75% of students knew which operation should be performed in biosafety cabinet; 28.28% of students could deal with waste according to the rules, 68.19% of students were able to identify warning signs of biological hazard; 92.82% of the students thought that laboratory biosafetyrelated courses should be set up. The overall awareness rate of laboratory biosafety knowledge and safety behavior was low, which were 42.65% and 41.96% respectively, juniors was higher than that of sophomores(P<0.05); in the aspect of chemical hazards and biological hazards, students with scientific research experience scored higher than those who did not participate in scientific research(all P<0.05).ConclusionMedical students’ cognition on knowledge of laboratory biosafety is not optimistic, it is imperative to strengthen the management of education and publicity of laboratory biosafety.