Genotypes and homology of measles virus isolated from 40 patients with measles in Beijing Xicheng District in 2014

Abstract:

ObjectiveTo understand the genotypes of measles virus isolated from patients with measles in Beijing Xicheng District in 2014, and timely find out imported measles cases. MethodsMeasles virus nucleic acid was identified with fluorescent polymerase chain reaction (PCR), then amplified by realtime PCR (RTPCR), amplified products were sequenced and analyzed. Results82 specimens from 81 suspected measles patients in Beijing Xicheng District in 2014 were taken, 63 were throat swab specimens and 19 were urine specimens. 70 cases were positive for measles virus nucleic acid, 40 of which were obtained gene sequence through sequencing, 37 were positive culture for measles virus. The phylogenetic tree analysis showed that gene sequences of 40 measles virus isolates were belonged to the same branch as H genotype representative strain Chin9322/H1a and MVi/Hunan.CHN/0.93/7/H1 recommended by World Health Organization.  The homology of nucleotide were 98%-98.9% and 96.9%-97.8% respectively, homology of amino acid were 97.3%-99.3% and 95.3%-98% respectively. ConclusionThe main genotype of measles cases in Beijing Xicheng District in 2014 was the local genotype (H1), surveillance of measles cases should be strengthened further to control and prevent the imported measles cases.