Molecular epidemiological characteristics of metallo-β-lactamase-producing Pseudomonas aeruginosa from Changsha

Abstract:

ObjectiveTo investigate the genotypes and epidemic of metallo-β-lactamase-(MBL)-producing Pseudomonas aeruginosa(P. aeruginosa) in Changsha. MethodsP. aeruginosa isolated from seven comprehensive hospitals in Changsha were collected and performed identification and antimicrobial susceptibility testing, phenotypes of MBL were detected with EDTA-disk synergy test and E-test, genotypes were determined by polymerase chain reaction (PCR), homology analysis were conducted by enterobacterial repetitive intergenic consensus PCR (ERICPCR). ResultsPreliminary screening by EDTA-disk synergy test and Etest showed that only 10 of 81 isolates were strong positive; PCR result showed that 18 isolates were positive for MBL, 11 of which were IMP-9-type MBL, 1 was IMP-1-type,and 6 were VIM-2-type.  SIM, SPM, GIM, and NDM-1-types were not found. ERIC-PCR showed that 12 strains of IMPproducing P. aeruginosa has multiple types, 6 VIM-2producing strains were of the same type.  ConclusionIMP-9 and VIM-2 are main genotypes in P. aeruginosa in Changsha．
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