Abstract:ObjectiveTo understand the specimen sources, clinical characteristics, and antimicrobial resistance of Burkholderia cepacia(B. cepacia) isolated from infected patients in intensive care unit(ICU), so as to provide reference for guiding rational use of antimicrobial agents.MethodsClinical data of patients with B. cepacia infection in an ICU between 2011 and 2014 were analyzed retrospectively, antimicrobial resistance of strains was analyzed.ResultsA total of 267 B. cepacia strains were isolated, the major specimen sources were sputum （80.15%,n=214）, blood（14.23%,n=38）,and urine（3.37%,n=9）. Antimicrobial susceptibility testing results revealed that B. cepacia had multiple resistance, and was naturally resistant to multiple clinically used antimicrobial agents, such as ampicillin, cefazolin, ampicillin/sulbactam, nitrofurantoin, and cefuroxime,resistant rates were all 100%; resistant rates to ceftazidime and levofloxacin were 4.12% and 3.00% respectively；resistant rate to compound sulfamethoxazole had increased tendency（χ2=5.885, P=0.015）.ConclusionIsolation of B. cepacia in ICU increased year by year, antimicrobial resistance is serious, management and targeted monitoring of prevention and control of healthcareassociated infection should be strengthened， antimicrobial agents should be chosen according to antimicrobial susceptibility testing results.