Detection of antimicrobial resistance and OXA genes in imipenemresistant Acinetobacter baumannii from Hohhot region

Abstract:

ObjectiveTo realize antimicrobial resistance and carrying status of  OXA carbapenemase among imipenemresistant Acinetobacter baumannii （IRAB）isolated from patients of Hohhot, so as to provide guidance for the prevention and control of healthcareassociated infection(HAI) caused by multidrugresistant Acinetobacter baumannii.  Methods49 IRAB isolates from 3 tertiary firstclass hospitals in Hohhot between January and December 2012 were collected,  antimicrobial susceptibility testing was performed by KirbyBauer disk diffusion method, four genotypes（blaOXA51like,blaOXA23like, bla OXA24like, bla OXA58like )  of  OXA carbapenemase were detected by polymerase chain reaction (PCR). ResultsAll 49 isolated IRAB strains were found to be highly resistant to antimicrobial agents（81.63%100.00%）except to minocycline（8.16%）;  blaOXA51like  was identified in 49 strains（100.00%），42(85.71%）of which also carried blaOXA23like gene ，blaOXA23lik and blaOXA51like were both found in three hospital, bla OXA24like and  bla OXA58like  weren’t found. ConclusionIRAB strains present multidrug resistance, resistant to minocycline is the lowest;  blaOXA23like is the main drugresistance mechanism of IRAB in Hohhot.