Abstract:ObjectiveTo investigate the influence of different handling factors in the hand hygiene compliance rate of health care workers’(HCWs). MethodsEight clinical departments in JulyDecember 2012 were selected for study, the baseline data of hand hygiene in July were investigated. In AugustOctober, 6 departments were provided free hand disinfectant, only 2 departments took comprehensive intervention measures; in NovemberDecember, all selected hospitals took intervention measures, but without being provided free hand disinfectant. Hand hygiene compliance rates among HCWs were compared. ResultsThe overall hand compliance rate of HCWs in JulyDecember 2012 was 59.50%,hand compliance rate of different months were significantly different (P<0.01), compliance rate was lowest in July(36.73%),after providing free hand disinfectant plus performing comprehensive intervention, hand compliance rates of different months increased obviously (57.20%-72.08%);When disinfectant was not provided free, compliance rate in each month still maintained a high level(65.20%-65.50%); compliance rates in AugustOctober were all higher than July ([45.41%-73.63%]vs [18.97%-47.17%])(all P≤0.05);except north section of intensive care unit (ICU), compliance rates in NovemberDecember (61.67%-72.73%)were both higher than July (both P<0.05).Healthcareassociated infection rate decreased from 3.39% in July to 1.75% in NovemberDecember (χ2=5.122,P=0.024).ConclusionProviding free hand disinfectant can promote HCWs’ hand hygiene compliance, repeated stressing hand hygiene awareness is the key measure to improve hand hygiene compliance.