Abstract:ObjectiveTo investigate the prevalence rate of healthcareassociated infection (HAI) in a hospital, so as to provide reference for making HAI control measures.MethodsThe crosssectional survey on HAI was carried out among all hospitalized patients at 0∶00—24∶00 on June 1,2010, June 20,2011 and April 16, 2012, respectively, surveyed data were analyzed.ResultsThe prevalence rate in 2010—2012 was 3.41%, 2.32% and 1.73% respectively（χ2=4.65,P＞0.05）; Univariate analysis revealed that risk factors for HAI were gender, age, urinary tract intubation, arteriovenous intubation, tracheotomy, the use of ventilator, and surgery;the top infection site was lower respiratory tract (59.65%), followed by urinary tract (15.79%), surgical site infection(10.53%), and gastrointestinal tract (8.77%); Antimicrobial use rate in three surveys was 57.95%, 43.17% and 40.23% respectively（χ2=54.25，P＜0.01）. A total of 26 pathogenic strains were isolated, Pseudomonas aeruginosa,Acinetobacter baumannii,Stenotrophomonas maltophilia were 11(42.31%)， 6（23.08%），3（11.54%) isolates respectively,Escherichia coli, Staphylococcus aureus and fungi were 2（7.69%） each. ConclusionPrevalence rate of HAI showed decreasing trend, proper measures should be taken, intervention in susceptible sites and management on antimicrobial use should be strengthened.