Drug resistance and detection of DHA1 gene of Ochrobactrum anthropi from infected children

Abstract:

ObjectiveTo evaluate drug resistance mechanisms of Ochrobactrum anthropi (O. anthropi) isolated from blood of children with septicemia. MethodsVITEK2 compact automatic machine was used to identify and analyze antimicrobial resistance of O. anthropi, and drugresistance gene AmpC, AmpR, DHA1, AcrA,RamA,OprD,TEM and SHV were determined by polymerase chain reaction (PCR) method, then PCR products were purified and sequenced. ResultsA total of 155 O. anthropi isolates were isolated, 30 of which were randomly selected for detection. Of 30 tested isolates, 20 were resistant to piperacillin / tazobactam, 16（80.00%，16/20） of which were detected AmpC/R genes, 2 （10.00%，2/20）were detected DHA1 gene. None of 30 isolates were amplified AcrA,RamA,OprD,TEM and SHV gene. According to the resistance to piperacillin/tazobactam, 155 isolates were divided into drugresistance group (126 isolates) and nondrugresistance group (29 isolates), drugresistance group were all resistant to ampicillin, firstthird generation cephalosporins, aztreonam, but resistant rates to levofloxacin, imipenem, meropenem, ciprofloxacin, amikacin and gentamycin were low （0.00%-1.59%）.ConclusionProduction of AmpC/R is the mechanism of multidrug resistance of O. anthropi,critically ill patients can choose imipenem. The detection of drug resistance of O. anthropi should be reinforced to prevent the prevalence of DHA gene among gramnegative bacteria.