In vitro study of Scutellaria Baicalensis Georgi water extraction on antirespiratory syncytial virus

Abstract:

Objective To evaluate the effect of antirespiratory syncytial virus of water extract liquid of Scutellaria Baicalensis Georgi,and to find the new drug for treatment of respiratory syncytial virus(RSV) infection.MethodsChinese medicine Scutellaria Baicalensis Georgi was turned into water extract liquid . Using in vitro cell culture technique ，the cytotoxicity of Scutellaria Baicalensis Georgi water extract was detected by 3（4，5dimethylthiazole2yl）2,5diphenyltetrazolium bromide (MTT) method. With ribavirin as the positive control drug, antiviral activity of Scutellaria Baicalensis Georgi water extract was detected with cell culture method.ResultsThe median cytotoxicity concentration(50% cytotoxicity concentration,CC50)of Scutellaria Baicalensis Georgi was 16.32 mg/mL, the median effective concentration (50% effective concentration,EC50) of the extraction of Scutellaria Baicalensis Georgi was 2.43 mg/mL. Scutellaria Baicalensis Georgi and ribavirin gave a dosedependent response in inhibiting RSV（P<0.05）.ConclusionScutellaria Baicalensis Georgi shows potent inhibition on RSV in vitro.