The MMP2 and TGFβ1 expression changes of rat liver fibrosis tissue influenced by Jiayijian

Abstract:

ObjectiveTo observe the effects of a traditional Chinese medicine Jiayijian on the matrix metalloproteinase2 (MMP2) and transforming growth factorbeta1 (TGFβ1) expression in experimental hepatic fibrosis rats.MethodsSixty male Wister rats were randomly divided into normal control group and liver fibrosis model group. Dimethylnitrosamine(DMN) was administered to induce liver fibrosis in the model group, and rats in model group were randomly divided into model control group and Jiayijian treatment groups with high concentration (22g/［kg·d］)and low concentration (5.5g/［kg·d］) 4 weeks after liver fibrosis had been induced. All rats were killed after 4 weeks intragastric administration of Jiayijian. The expression of MMP2 and TGFβ1 of rat liver tissue were detected by immunohistochemical method.ResultsThe expression of TGFβ1 in high and low concentrations of Jiayijian treatment groups was significantly lower than that in the model control group (P<0.05), and the expression of TGFβ1 in the high concentration of Jiayijian treatment group was significantly lower than the low concentration of Jiayijian treatment group (P<0.05), but there was no significant difference in the expression of MMP2 between high and low concentrations of Jiayijian treatment groups and the model control group (P>0.05).ConclusionJiayijian can downregulate the expression of TGFβ1 of experimental hepatic fibrosis model rat’s liver tissue, which is the one of the mechanisms of antiliverfibrosis.