Function of dendritic cell to secrete interleukin12 in patients with chronic hepatitis B

Abstract:

［Abstract］ObjectiveTo study the immune function of dendritic cell (DCs) in HBVinfected patients.MethodsHBV DNA in DCs of 12 chronic hepatitis B (CHB) patients with serum positive HBV DNA and 10 CHB patients with serum negative HBV DNA were detected by fluorescent quantitative polymerase chain reaction, the  function of secreting interleukin12(IL12) by DCs under different titers of HBV was compared, 10 persons with negative HBV markers and normal liver function were as control.ResultsHBV DNA in DCs was detected in 7 of 12 (58.33%) CHB patients with positive HBV DNA. The levels of IL12 in DCs supernatants of serum positive HBV DNA group were obviously  lower than  that of HBV DNA negative and healthy control group（both P<0.01）; The expression levels of IL12 in DCs HBV DNA positive group was obviously lower than that of DCs HBV DNA negative group and healthy control group（ both P<0.01）.ConclusionThe DCs derived from peripheral blood in patients with CHB can  be infected by HBV, the secretion function of IL2 by HBVinfected DCs decreased significantly