Abstract:Objective To analyze the epidemiological characteristics of methicillin resistant Staphylococcus aureus (MRSA) in nosocomial infection with pulsed field gel electrophoresis (PFGE) and PCR SCCmec typing.Methods Forty strains of MRSA collected from Tianjin Nankai Hospital between January, 2007 and December, 2008 were performed antimicrobial susceptibility analysis, PFGE and SCCmec genotyping.Results Antimicrobial susceptibility patterns of 40 strains of MRSA were highly similar, isolates were classified into AE five types by PFGE typing，type A, B, C, D, E were 21, 8, 4, 6, 1 strains respectively； SCCmec typing showed that 1 strain was SCCmecⅠ,33 were SCCmecⅢ, 1 was SCCmecⅣ，and 5 were SCCmecV. Sporadic infection of MRSA occurred in the hospital during the studied period. Conclusion Epidemiological analysis indicates that PFGE typing can identify the genetic relationship between isolates, SCCmec typing can suggest whether MRSA isolates are hospitalacquired .Combination of two genotyping methods can show characteristics of epidemic strains, and provide reference for nosocomial infection surveillance.