Detection and clinical significance of HIV1 antibody in FREE

Abstract:

Detection and clinical significance  of HIV1 antibody in urineGUO Jingjing1, XU Dan2, WANG Min2, XIE Weiwei3, LIU Wenen1(1 Xiangya Hospital, Central South University, Changsha 410008, China; 2 Infectious Disease Hospital of Changsha,  Changsha 410011, China; 3 Department of Medical Laboratory of Xiangya School of Medicine,  Central South University, Changsha 410013, China)［Abstract］ObjectiveTo evaluate the clinical significance of detecting antiHIV1 antibody in urine samples.  MethodsAntiHIV1 antibody was  tested in serum and urine samples from 59 HIVinfected persons diagnosed by Provincial Center for Disease Control and Prevention and 30 healthy controls with  ELISA； Taking the results of the counterpart serum samples tested as standard, the sensitivity and specificity of  antiHIV1 antibody in urine samples were calculated.ResultsAmong 59 HIVinfected cases, antiHIV1 antibody positive results of serum test were observed in all cases, while 53 urine samples showed positive and 6 ones were negative. None of the serum samples showed antiHIV1 antibody positive in the control group, but 1 urine sample was positive, which was determined to be negative by serum enzymelinked immunosorbent assay and electroselenium assay. The sensitivity and specificity for the urine ELISA were 89.83% and 96.67% respectively. ConclusionThe results indicate that urine antiHIV1 antibody can be tested to screen HIV infection in high risk population, if serum samples are unavailable.