Immune response of T lymphocytes in patients with pulmonary tuberculosis combined with chronic hepatitis B FREE

Abstract:

Immune response of T lymphocytes in patients with pulmonary tuberculosis combined with chronic hepatitis B GUO Haoyu1, LIU Hongbo2, ZHANG Chengguo1 (1 Changsha Central Hospital, Changsha 410004, China; 2 Xiangya Hospital, Central South University, Changsha 410008, China )［Abstract］ObjectiveTo study the serum levels of Th1 lymphocyte factor and the expression pattern of Th1 and Th2 in patients with pulmonary tuberculosis (PT)combined with chronic hepatitis B (CHB)．MethodsSerum levels of tumor necrosis factorα(TNFα),interferonγ(IFNγ),interleukin12 (IL12) in 21 patients with PT combined with positive HBsAg, 30 simple PT patients and 30 healthy controls were detected with enzymelinked immunosorbent assay (ELISA) ;and the expression pattern of CD4+T lymphocytes,Th1 and Th2 cells in peripheral blood were detected by flow cytometry．ResultsThe serum levels of TNFα in patients with simple PT and PT coinfected with CHB were both higher than that in healthy group (both P<0.01)；the serum levels of IFNγ and IL12 in patients with simple PT were both lower than that in healthy group (both P<0.05)；the serum levels of IFNγ and IL12 in patients with PT coinfected with CHB were both lower than that in simple PT patients (both P<0.01).The expression of CD4+ T lymphocytes and Th1 cells in simple PT patients were significantly lower than that in healthy group (P<0.05，P<0.01)；The expression of Th2 cells in simple PT patients was significantly higher than that in healthy group (P<0.05)；The expression of CD4+ T lymphocytes and Th1 cells in PT coinfected with CHB patients were significantly lower than that in simple PT patients (P<0.05，P<0.01).ConclusionPatients with PT showed lower expression of Th1 and higher expression of Th2; patients with PT coinfected with CHB showed further lower expression of Th1 and higher expression of Th2.