Detection of blood HBV DNA in blood donors with HBsAg(-)/antiHBc(+) by realtime fluorescence PCR

Abstract:

ObjectiveTo evaluate the risks of bloodtransmitted HBV through blood donors with  HBsAg(-)/antiHBc(+),so as to improve  better blood screening mode and ensure blood safety in clinical application. MethodsBlood HBV DNA in serologically screened negative blood samples  pooled at 8×45 μL size were detected by realtime fluorescence PCR, and  PCR negative samples were tested for HBsAg, antiHBs, HBeAg, antiHBe and antiHBc by ELISA. All HBsAg(-), HBV DNA(-) and antiHBc positive samples were detected by  realtime fluorescence PCR individually for HBV DNA, HBV DNA positive samples were analysed by quantitative fluorescence PCR.ResultsAmong 12 552 seronegative samples, 2 were detected HBsAg(-)/HBV DNA(+), the positive rate was 0.02%. Among 614 PCR negative samples, 320 were positive antiHBc, and one of which was positive HBV DNA, the positive rate was 0.31%. ConclusionDonors with HBsAg(-)/antiHBc(+) remain potential risk for HBV transmission, and nucleic acid amplication technique can  implement  and improve blood safety.

Key words:

blood transfusion; blood donor; nucleic acid amplification technique; realtime fluorescence PCR; hepatitis B virus;antiHBc