大鼠脓毒症肺损伤与中性粒细胞载脂蛋白的相关性研究

Correlation between lung injury and human neutrophil lipocalin in rats with sepsis

摘要:

目的 探究大鼠脓毒症肺损伤与中性粒细胞载脂蛋白(HNL)的相关性。方法 45只大鼠按照随机方法分为对照组、sham组及模型组,模型组建立脓毒症肺损伤大鼠模型,sham组开腹后仅游离盲肠后关腹,不进行结扎和穿刺,对照组为健康大鼠。使用试剂盒检测各组大鼠血清白细胞介素(IL)-6、IL-1β、肿瘤坏死因子-α(TNF-α)及HNL;血气分析仪检测氧合指数;计算肺组织湿/干比;HE染色观察肺组织病理形态,计算肺损伤评分。结果 对照组及sham组12、24、36 h的IL-6、IL-1β、TNF-α、HNL水平、氧合指数、肺组织湿/干比、肺损伤评分比较,差异均无统计学意义(均P＞0.05);与sham组相比,模型组12、24、36 h的IL-6、IL-1β、TNF-α、HNL水平、氧合指数、肺组织湿/干比、肺损伤评分均升高,差异均有统计学意义(均P＜0.05)。对照组大鼠12、24、36 h肺组织结构正常,且未见水肿,sham组大鼠12、24、36 h肺组织仅有少量炎症细胞,模型组大鼠12、24、36 h肺组织结构严重破坏,肺泡萎陷,炎症细胞严重浸润,但随着时间延长病理改善。脓毒症肺损伤大鼠中,HNL与IL-6、IL-1β、TNF-α、肺部湿/干比、肺损伤评分呈现正相关性(均P＜0.05),与氧合指数呈现负相关性(P＜0.05)。结论 脓毒症肺损伤大鼠中HNL显著升高,炎症反应严重,肺组织湿/干比、肺损伤加重,氧合指数降低,且HNL水平与IL-6、 IL-1β、TNF-α水平、肺组织湿/干比、肺损伤呈正相关性,与氧合指数呈负相关性。

Abstract:

Objective To explore the correlation between lung injury and human neutrophil lipocalin (HNL) in rats with sepsis. Methods 45 rats were randomly divided into a control group, a sham group, and a model group. The model group was to construct a rat sepsis lung injury model, the sham group was to free cecum laparotomy and then close abdomen without ligation and puncture, and the control group was with healthy rats. Serum interleukin (IL)-6 , IL-1 β, tumor necrosis factor-α (TNF-α), and HNL in each group of rats were detected with reagent kit. Oxygenation index was detected by blood gas analysis system. Lung wet/dry (W/D) ratio was calculated. Pathological morphology of lung tissue was observed by HE staining, and lung injury score was calculated. Results There were no significant differences in IL-6, IL-1β, TNF-α, HNL levels, oxygenation index, lung tissue wet/dry ratio, and lung injury score between the control group and the sham group at 12, 24 and 36 hours (all P>0.05). Compared with the sham group, IL-6, IL-1β, TNF-α, HNL levels, oxygenation index, lung tissue wet/dry ratio, and lung injury score in the model group increased at 12, 24 and 36 hours, with significant differences (all P<0.05). At 12, 24 and 36 hours, lung tissue structure of rats was normal in the control group, with no edema observed; there were only a few inflammatory cells in the lung tissue of rats from the sham group; while in the model group, lung tissue structure of rats was severely injured, pulmonary alveoli collapsed, and inflammatory cells were severely infiltrated, but pathology improved with time. In rats with sepsis and lung injury, HNL was positively correlated with IL-6, IL-1β, TNF-α, lung wet/dry ratio, and lung injury scores (all P<0.05), while negatively correlated with oxygenation index (P<0.05). Conclusion In rats with sepsis lung injury, HNL increases significantly, with severe inflammation and aggravation in lung tissue wet/dry ratio and lung injury, while oxygenation index decreases. HNL level is positively correlated with IL-6, IL-1β, TNF-α levels, lung tissue wet/dry ratio and lung injury, but negatively correlates with oxygenation index.