慢性乙型肝炎患者外周血SOCS3 mRNA的表达及其与Th17的关系

Expression of SOCS3 mRNA in peripheral blood of patients with chronic hepatitis B and its relationship with Th17

摘要:

目的 研究慢性乙型肝炎(CHB)患者外周血辅助性T细胞17(Th17)以及细胞因子信号转导抑制因子3 (SOCS3) mRNA的表达状况，探索CHB患者SOCS3 mRNA表达与Th17的关系。 方法 选取2021年2—8月于某院门诊就诊的30例CHB患者为研究对象，并选取同期正常体检者15例为对照组。采用流式细胞术(FCM)检测外周血Th17细胞频数，酶联免疫吸附试验(ELISA)检测血清细胞因子IL-17A和IL-23表达水平，实时荧光定量逆转录聚合酶链反应(qRT-PCR)法测定外周血SOCS3、维甲酸相关孤儿核受体γt(RORγt) mRNA表达水平，并比较两组患者的检测结果。 结果 CHB患者外周血Th17细胞频数及其效应分子IL-17A、IL-23表达水平高于对照组(均P＜0.05)，Th17细胞频数、IL-17A与HBV DNA水平之间存在正相关(r值分别为0.570、0.563，均P＜0.005)。CHB患者外周血SOCS3、RORγt mRNA表达水平高于对照组(均P＜0.05)，两者与HBV DNA水平正相关(r值分别为0.662、0.561，均P＜0.05)。CHB患者SOCS3 mRNA与RORγt mRNA、Th17细胞频数、IL-17A之间存在正相关(r值分别为0.552、0.626、0.826，均P＜0.05)。 结论 CHB患者外周血SOCS3 mRNA的异常高表达，可能通过调节RORγt mRNA的表达来影响Th17的分化及其效应分子的分泌。

Abstract:

Objective To analyze the expression of T helper 17 cells (Th17) and suppressor of cytokine signaling 3 (SOCS3) mRNA in peripheral blood of patients with chronic hepatitis B (CHB), and explore the relationship between SOCS3 mRNA expression and Th17 in patients with CHB. Methods 30 CHB patients who were admitted to the outpatient department of a hospital from February to August 2021 were selected as the research objects, and 15 healthy subjects were selected as control during the same period. Frequency of Th17 cells in peripheral blood was conducted with flow cytometry (FCM), expression levels of serum cytokines interleukin-17A (IL-17A) and IL-23 were detected with enzyme-linked immunosorbent assay (ELISA); mRNA expression levels of SOCS3 and retinoid-related orphan receptor gamma t (RORγt) in peripheral blood were determined by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), results of two groups of patients were compared. Results The frequency of Th17 cells and expression levels of its effector molecules IL-17A and IL-23 in peripheral blood of patients with CHB were higher than those in control group (all P < 0.05), there were positive correlations between Th17 cell frequency, IL-17A and HBV DNA levels (r=0.570, r=0.563, both P < 0.005). The expression levels of SOCS3 and RORγt mRNA in peripheral blood of CHB patients were both higher than those of healthy control (both P < 0.05), they were also positively correlated with HBV DNA levels (r=0.662, r=0.561, both P < 0.05). In CHB patients, SOCS3 mRNA was positively correlated with RORγt mRNA, Th17 cell frequency and IL-17A (r=0.552, r=0.626, r=0.826, all P < 0.05). Conclusion The abnormal expression of SOCS3 mRNA in peripheral blood of patients with CHB may affect the differentiation of Th17 and the secretion of its effector molecules by regulating the expression of RORγt mRNA.