三种不同含漱液对口腔超声洁治时空气中菌落数的影响

Effect of three different gargling liquid on bacterial colonies in air during oral ultrasound scaling

摘要:

目的 评价牙周炎患者进行口腔超声洁治时使用不同含漱液对空气中菌落数的影响。方法 分别选取轻、中、重度慢性牙周炎患者各54例，再随机将同种程度的牙周炎患者各分成3组，每组18例。洁治前分别给轻、中、重度牙周炎的各组患者用金银花液（含漱液A）、3%双氧水（含漱液B）、生理盐水（含漱液C）进行含漱。对超声洁治开始0、30 min及结束后10、20 min的空气样本进行细菌培养和菌种鉴定。结果 三因素析因设计资料的方差分析结果显示：牙周炎程度、含漱液种类及采样时段三个因素存在交互作用（F=2.666，P=0.002）。空气菌落数比较：洁治开始时，轻、中、重度各组患者中使用不同含漱液时空气菌落数比较，差异均无统计学意义（均P>0.05）。洁治开始30 min，使用相同漱口液患者中均为轻度牙周炎患者的空气菌落数最低，重度患者最高，各组患者使用含漱液A、B时的空气菌落数较使用含漱液C低。各采样点比较，患者头部右侧采样点菌落数最高，差异均有统计学意义（均P<0.05）。结束后20 min，各组空气菌落数与洁治前比较，差异均无统计学意义（均P>0.05）。空气中检出菌种主要为草绿色链球菌（32.53%）、凝固酶阴性葡萄球菌（24.56%）、丝状真菌（18.48%）等。结论 口腔超声洁治时空气中存在多种条件致病菌，菌落数与牙周炎程度呈正相关。使用金银花液含漱对降低洁治中、后的空气菌落数有良好效果。

Abstract:

Objective To evaluate the effect of different gargling liquid on colony number in air during oral ultrasound scaling for patients with periodontitis. Methods Patients with mild(n=54), moderate(n=54), and severe chronic periodontitis(n=54) were selected, then patients with same degree of periodontitis were randomly divided into three groups, with 18 in each group. Before scaling, patients in each group of mild, moderate, and severe periodontitis were given honeysuckle liquid (gargle A), 3% hydrogen peroxide (gargle B), and normal saline (gargle C) respectively. Bacterial culture and identification of air specimens were conducted at 0 and 30 minutes after the beginning of ultrasonic scaling as well as 10, 20 minutes after the end of ultrasonic scaling. Results Variance analysis of three-factor factorial design data showed that degree of periodontitis, types of gargling liquid, and sampling time had interaction (F=2.666, P=0.002). Comparisons of air colonies:At the beginning of scaling, there was no significant difference in colony number of air around patients using different gargling liquid in mild, moderate and severe groups (all P>0.05). Among patients using the same gargling liquid at 30 minutes after the beginning of scaling, colony number of air was the lowest around patients with mild periodontitis and the highest around patients with severe periodontitis, colony numbers of air around patients with gargle A and B were lower than that of patients with gargle C. Comparison of each sampling points showed that colony number of air around the right side of patients' head was highest, differences were all statistically significant (all P<0.05). 20 minutes after the end of scaling, there was no significant difference in colony number among groups compared before scaling (all P>0.05). The main isolated strains in the air were Streptococcus viridans(32.53%), coagulase negative staphylococcus(24.56%), and filamentous fungi(18.48%). Conclusion There are variety of opportunistic pathogens in the air during oral ultrasound scaling, and the number of colonies is positively correlated with the degree of periodontitis. honeysuckle liquid has a good effect on reducing the number of air colonies during and after scaling.