HIV-1/HBV共感染患者HBV病毒基因组前S区、BCP区和前C区的变异

Variation in preS region, BCP region, and preC region of HBV virus genomes from HIV1/HBV coinfected patients

摘要:

目的探讨乙型肝炎病毒(HBV)合并人类免疫缺陷病毒1型(HIV1)感染者和HBV单独感染者HBV基因组三个重点区域的变异。方法收集湖南省HIV1/HBV合并感染患者（试验组）和HBV单独感染患者（对照组）血清各40份，进行HBV全基因组扩增及测序，并对突变位点进行分析。结果有59份血清HBV成功分型和测序，其中试验组21份，对照组38份，试验组与对照组HBV载量值和不同基因型比较,差异均有统计学意义(均P<0.05 )。试验组4例患者血清标本HBV在前S区22个氨基酸发生变异，12例在前C区和BCP区45个核苷酸发生突变，试验组和对照组前S区氨基酸总体缺失突变率分别为0.60%、0.64%，差异无统计学意义(χ2=0.042, P>0.05)。试验组与对照组前C区和BCP区各个主要突变位点变异发生率比较，差异无统计学意义 (均P>0.05)，试验组和对照组前C区和BCP区总体变异发生率分别为1.36%、1.73%，差异无统计学意义(χ2=1.920, P>0.05)。结论共感染患者的HBV变异水平与单感染患者基本一致，短时间内HIV1暂未对HBV的进化突变造成显著影响。

关键词:

人类免疫缺陷病毒1型;乙型肝炎病毒;合并感染;突变

Abstract:

ObjectiveTo evaluate variation of three key regions of HBV genome in patients with coinfection of hepatitis B virus (HBV) and human immunodeficiency virus type 1 (HIV1) as well as those only infected with HBV.MethodsForty serum specimens from patients with coinfection of HIV1/HBV(trial group) as well as 40 serum specimens from those only infected with HBV(control group) in Hunan Province were collected, the wholegenome of HBV was amplified and sequenced, the mutation sites were analyzed.ResultsHBV in serum of 59 cases were successfully genotyped and sequenced, 21 of which were from trial group and 38 from control group, there was significant difference in HBV load and genotype between trial group and control group (both P<0.05). In trial group, 4 patients were detected 22 amino acid mutations in the preS region of HBV in serum specimen, 12 were detected 45 nucleotide mutations in preC and basic core promoter (BCP) regions, the overall deletion mutation rates of amino acid in preS region of trial group and control group were 0.60% and 0.64% respectively, with no significant difference (χ2=0.042, P>0.05). There were no significant difference in mutations in key sites of preC and BCP regions between trial group and control group (both P>0.05), the overall variation rates in preC and BCP regions of trial group and control group were 1.36% and 1.73% respectively, difference was not significant (χ2=1.920, P>0.05).ConclusionHBV mutation in coinfected patients is basically the same as that in singleinfected patients, HIV1 has not obvious affect on the evolution of HBV mutation during a short time.

Key words:

human immunodeficiency virus type 1; hepatitis B virus; coinfection; mutation