人员活动和空气净化器对支气管镜室空气微生物和颗粒物的影响

Effect of human activities and air purifier on airborne microorganisms and particulate matter in a bronchoscopy room

摘要:

目的评价人员活动和空气净化器对支气管镜室空气微生物及颗粒物的影响。方法依照支气管镜室有无人员活动和空气净化器，将实验分成四组：动态无净化组、动态净化组、静态无净化组、静态净化组，在五个不同时间点（0、0.5、1、2、4 h）对室内空气进行采样和分析，用浮游菌法采集空气中的微生物并培养、计数，用DT9881M激光尘埃粒子计数器检测颗粒物浓度,统计方法采用析因设计的方差分析。结果动态无净化组的细菌、真菌、总微生物（细菌+真菌）、PM2.5和PM2.5～10.0菌落数/浓度分别为（113.53±7.78）CFU/m3、（89.67±7.17）CFU/m3、（203.20±10.92）CFU/m3、（86 557.20±4 158.29）个/m3和（659.69±38.91）个/m3,静态无净化组分别为（84.33±3.65）CFU/m3、（65.00±2.65）CFU/m3、（149.33±4.98）CFU/m3、（45 812.64±1 279.61）个/m3和（189.15±4.64）个/m3，动态净化组分别为（84.80±8.08）CFU/m3、（90.40±5.50）CFU/m3、（175.20±9.22）CFU/m3、（49 336.38±2 039.16）个/m3和（218.36±7.02）个/m3，静态净化组分别为（67.80±5.63）CFU/m3、（38.27±3.70）CFU/m3、（106.07±6.76）CFU/m3、（29 772.53±2 212.93）个/m3和（124.80±7.16）个/m3，细菌、总微生物、PM2.5、PM2.5～10.0菌落数/浓度动态组高于静态组，无净化组高于净化组（均P＜0.05），真菌菌落数动态无净化组高于静态无净化组，静态净化组低于静态无净化组（均P＜0.05），动态净化组与无净化组间无明显差异（P=0.936）。结论人员活动增加支管镜室空气微生物和颗粒物的菌落数/浓度，空气净化器能降低支气管镜室空气中的细菌、总微生物和颗粒物的菌落数/浓度。

Abstract:

ObjectiveTo evaluate the effect of personnel activities and air purifiers on airborne microorganisms and particulate matter in bronchoscopy room.MethodsAccording to whether there was personal activity and air purifier in the bronchoscopy room, the experiment was divided into four groups：dynamic nonpurification group, dynamic purification group, static nonpurification group, and static purification group, indoor air samples were collected and analyzed at five different time points (0, 0.5, 1, 2, 4 h), microorganisms in the air were collected by planktonic method, then cultured and counted, concentration of particulate matter was determined by DT9881M laser dust particle counter, variance analysis of factorial design was used for statistical analysis. ResultsColony count/concentration of airborne bacteria, fungi, total microorganisms (bacteria+fungi), PM2.5, and PM2.5-10.0 in dynamic nonpurification group were (113.53±7.78) CFU/m3，(89.67±7.17) CFU/m3，(203.20±10.92) CFU/m3，(86 557.20±4 158.29) counts/m3，and (659.69±38.91) counts/m3 respectively, in static nonpurification group were (84.33±3.65) CFU/m3, (65.00±2.65)CFU/m3, (149.33±4.98) CFU/m3, (45 812.64± 1 279.61) counts/m3, and (189.15±4.64) counts/m3 respectively, in dynamic purification group were (84.80±8.08) CFU/m3，(90.40±5.50) CFU/m3，(175.20±9.22) CFU/m3，(49 336.38±2 039.16) counts/m3, and (218.36±7.02) counts/m3 respectively，in static purification group were (67.80±5.63) CFU/m3, (38.27±3.70) CFU/m3, (106.07±6.76) CFU/m3, (29 772.53±2 212.93) counts/m3, and (124.80±7.16) counts/m3 respectively. Colony count/concentration of airborne bacteria, total microorganisms, PM2.5, and PM2.5-10.0 in dynamic group were all higher than those in static group, nonpurification group were higher than purification group(both P<0.05)，colony count of fungi in dynamic nonpurification group was higher than static nonpurification group, in static purification group was lower than static nonpurification group(both P<0.05)，there was no significant difference between dynamic purification group and dynamic nonpurification group （P=0.936）.ConclusionPersonal activities can increase colony count/concentration of microorganisms and particulate matter in bronchoscopy room, air purifier can reduce the bacteria, total microbial count, and particulate matter in the air of bronchoscopy room.