不同细菌所致小鼠血流感染模型中MIP-1β、MIP-2和IL-12p70的表达及变化规律

Expression and variation of MIP-1β, MIP-2, and IL-12p70 in mouse models with bloodstream infection caused by different bacteria

摘要:

目的探讨4种血流感染常见细菌致小鼠血流感染后MIP1β、MIP2和IL12p70的表达及变化规律。方法建立金黄色葡萄球菌、粪肠球菌、大肠埃希菌和肺炎克雷伯菌CD1(ICR)小鼠血流感染模型，采用Luminex液相芯片系统检测各实验组和PBS对照组小鼠感染后0.5、1、3、6、12、24和48 h MIP1β、MIP2和IL12p70的含量。结果MIP1β的浓度在细菌入血后1 h时明显升高，金黄色葡萄球菌、粪肠球菌、大肠埃希菌、肺炎克雷伯菌组和对照组浓度分别为（134.5±18.3）、（61.5±15.4）、（3 354.0±809.0）、（6 888.4±1 100.2）和（28.9±4.6）pg/mL；IL12p70达峰值时的浓度分别为（389.3±118.1）、（127.6 ±10.0）、（42.2±3.5）、（62.8±8.4）和（4.8±0.3）pg/mL。MIP1β、MIP2的浓度在大肠埃希菌组和肺炎克雷伯菌组高于其他实验组和对照组（均P<0.01），而IL12p70的浓度在金黄色葡萄球菌组和粪肠球菌组中高于大肠埃希菌组、肺炎克雷伯菌组和对照组（均P<0.01）。结论大肠埃希菌组和肺炎克雷伯菌组MIP1β和MIP2的浓度高于金黄色葡萄球菌组和粪肠球菌组，而金黄色葡萄球菌组和粪肠球菌组IL12p70的浓度高于大肠埃希菌组和肺炎克雷伯菌组。联合检测多种细胞因子或趋化因子在预测革兰阳性或革兰阴性菌感染方面有一定价值，且可能为感染早期指导治疗提供依据。

Abstract:

ObjectiveTo investigate the expression and variation of MIP1β, MIP2, and IL12p70 in mice with bloodstream infection caused by 4 kinds of bacteria.MethodsCD1 (ICR) mouse models of bloodstream infection with Staphylococcus aureus (S. aureus), Enterococcus faecalis(E. faecalis), Escherichia coli(E. coli), and Klebsiella pneumoniae(K. pneumoniae) were established. After mice in each trial group and PBS control group were infected by bacteria for 0.5h, 1h, 3h, 6h, 12h, 24h, and 48h, concentrations of MIP1β, MIP2, and IL12p70 were detected by Luminex liquid suspension chip system.ResultsConcentrations of MIP1β increased significantly 1h after bacteria was in blood, S. aureus, E. faecalis, E. coli, K. pneumoniae, and control groups were (134.5±18.3), (61.5±15.4), (3 354.0±809.0), (6 888.4±1 100.2), and (28.9±4.6) pg/mL respectively; the peak values of IL12p70 were (389.3±118.1), (127.6±10.0), (42.2±3.5), (62.8±8.4), and (4.8±0.3) pg/mL respectively. Concentrations of MIP1β and MIP2 in E. coli and K. pneumoniae groups were significantly higher than other trial groups and control group (all P<0.01), while concentrations of IL12p70 in S. aureus and E. faecalis groups were both significantly higher than E. coli, K. pneumoniae, and control groups (all P<0.01).ConclusionConcentrations of MIP1β and MIP2 in E. coli and K. pneumoniae groups were both significantly higher than those in S. aureus and E. faecalis groups, while concentrations of IL12p70 in S. aureus and E. faecalis groups were both significantly higher than those in E. coli and K. pneumoniae groups. The combination detection of multiple cytokines or chemokines are valuable in predicting grampositive or gramnegative bacterial infection, and can provide basis for treatment of early infection.