硝酸镓对临床分离金黄色葡萄球菌生物膜的体外清除作用

In vitro clearance effects of gallium nitrate on biofilms of clinically isolated Staphylococcus aureus

摘要:

目的研究新型抗菌剂硝酸镓对金黄色葡萄球菌生物膜的清除作用，探索抑制细菌生物膜的新方法。方法采用结晶紫染色法，对临床分离的14株金黄色葡萄球菌进行生物膜阳性菌株筛选，检测硝酸镓对生物膜阳性菌株的最低抑菌浓度（MIC）和对生物膜的清除作用。结果14株金黄色葡萄球菌中，生物膜阳性菌株9株。硝酸镓对金黄色葡萄球菌ATCC 25923、生物膜阴性菌株以及9株生物膜阳性菌株的MIC均为16 μg/mL。硝酸镓对金黄色葡萄球菌早期生物膜的清除率为（86.53 ±0.96）%，高于对晚期生物膜的清除率［（62.54±1.53）%］ （t=35.699，P<0.001）。结论硝酸镓具有抑制金黄色葡萄球菌增殖，清除其生物膜的作用，有望成为防治金黄色葡萄球菌感染的新型药物。

Abstract:

ObjectiveTo study the effect of new type antiseptic gallium nitrate on the clearance of Staphylococcus aureus (S. aureus) biofilm, and  explore new methods for suppressing bacterial biofilm formation. MethodsBiofilm positive strains were screened among 14 clinically isolated S. aureus strains by crystal violet staining method, minimal inhibitory concentration (MIC) of gallium nitrate for biofilm positive strains and effect of gallium nitrate on the clearance of biofilm were measured. ResultsOf 14 S.aureus isolates, 9 were biofilm positive strains; gallium nitrate MICs for S. aureus ATCC 25923, biofilmnegative strain, and 9 biofilm positive strains were all 16 μg/mL; the clearance rate of gallium nitrate for early biofilm of S.aureus was significantly higher than mature biofilm (［86.53±0.96］% vs ［62.54±1.53］ %, t=35.699,P<0.001). ConclusionGallium nitrate can inhibit growth of S. aureus strains and clear biofilm, it can be applied in the prevention and control of  S. aureus infection.