肠杆菌科细菌KPC型碳青霉烯酶的研究

Study on Klebsiella pneumoniae carbapenemase in isolates of Enterobacteriaceae

摘要:

目的了解某院临床分离的肠杆菌科细菌产KPC型碳青霉烯酶情况及其基因型别。方法收集该院2009—2010年临床分离的肠杆菌科细菌1 801株，经药敏试验筛选出耐药性高的菌株，采用改良Hodge试验和聚合酶链反应（PCR）扩增检测细菌产KPC型碳青霉烯酶情况，并测序分析其基因型别。结果1 801株肠杆菌科细菌中，有783株（43.48%）对第三代头孢菌素耐药，其中4株还对碳青霉烯类抗菌药物耐药；改良Hodge试验初筛出2株耐药菌株，经PCR扩增证实为碳青霉烯酶blaKPC2基因。结论该院已出现产KPC2型碳青霉烯酶耐药基因的肠杆菌科细菌，临床与实验室应加强监测和控制。

Abstract:

ObjectiveTo investigate the Klebsiella pneumoniae carbapenemase (KPC) production in clinical isolates of Enterobacteriaceae in a hospital.Methods1 801 clinical Enterobacteriaceae isolates were collected from this hospital from 2009 to 2010. Highly drugresistant strains were screened by antimicrobial susceptibility testing, KPCtype carbapenemase was detected with the modified Hodge testing and polymerase chain reaction (PCR), and DNA sequencing was performed to confirm carbapenemase genotypes.ResultsOf 1 801 Enterobacteriaceae isolates, 783(43.48%) were resistant to the third cephalosporins, 4 of which were also resistant to carbapenem; 2 isolates were screened as drugresistant strains by Hodge testing, and blaKPC2 gene was identified in this 2 isolates by PCR.ConclusionKPC2 Enterobacteriaceae strains with carbapenemaseproducing drugresistant gene have emerged in this hospital, monitor and control should be strengthened.