1株沙门样大肠埃希菌的分离与鉴定

Isolation and identification of one Salmonellalike Escherichia coli isolate

摘要:

目的对1株与沙门菌抗血清发生交叉凝集的肠道菌分离株进行鉴定。方法分别通过生化培养、抗血清凝集试验与16S rRNA序列比对分析,对该菌株进行鉴定。结果该菌株的分离培养、生化代谢特征均与大肠埃希菌一致，但能与沙门菌属多型、群特异性抗血清发生凝集，与大肠埃希菌抗血清无凝集反应。序列分析结果表明,该菌株16S rRNA与大肠埃希菌一致，与沙门菌属存在明显差异。结论本实验分离获得1株具有沙门菌血清凝集特征的大肠埃希菌。

关键词:

大肠埃希菌;沙门菌属;交叉凝集;16S rRNA

Abstract:

ObjectiveTo identify and characterize one Enterobacteriaceae isolate which could crossagglutinate with Salmonella diagnostic antiserum.MethodsPhenotype and genotype of the isolate were determined by culture，metabolic assay, agglutination reaction， and 16S rRNA sequence phylogenetic analysis, respectively.ResultsThe isolate was consistent with Escherichia coli on culture and biochemical features, but could crossagglutinate with multiple types or groupspecific Salmonella antiserum rather than Escherichia coli antiserum. 16S rRNA sequence analysis revealed that the isolate was identical with Escherichia coli, but was obviously different from Salmonella spp.ConclusionOne Escherichia coli isolate which can crossagglutinate with Salmonella diagnostic antiserum has been obtained in this study.

Key words:

Escherichia coli;Salmonella spp.; crossagglutination;16S rRNA