8株医院血流感染肺炎克雷伯菌的PFGE分型

PFGE typing of 8 Klebsiella pneumoniae strains causing nosocomial bloodstream infections

摘要:

目的应用脉冲场凝胶电泳（PFGE）技术对某医院同一外科病房临床分离的8株肺炎克雷伯菌进行同源性分析，为医院感染控制提供依据。方法对此8株肺炎克雷伯菌，采用微量肉汤稀释法和KB法进行药物敏感试验，并以PFGE技术进行基因分型。结果药物敏感试验结果显示，除菌株Kp1外，菌株Kp2等其他7株肺炎克雷伯菌对11种抗菌药物具有相同的药敏谱，且为多重耐药菌；PFGE分析亦显示此7株菌为同一谱型。结论此外科病房临床分离的8株肺炎克雷伯菌中有7株来源于同一克隆株，提示可能是一次医院感染暴发流行。

Abstract:

ObjectiveTo analyze 8 strains of Klebsiella pneumoniae isolated from the surgical ward in a hospital by pulsedfield gel electrophoresis(PFGE), so as to provide scientific evidence for the control of nosocomial infection. MethodsAntimicrobial susceptibility test of Klebsiella pneumoniae was performed by microbroth dilution and KirbyBauer method, genotyping was performed with PFGE.ResultsAntimicrobial susceptibility test showed that except the strain Kp1, 7 clinical strains were the same sensitivity to 11 antimicrobial agents, and they were all multidrug resistant； PFGE showed that 7 strains were the same pattern. ConclusionSeven of 8 Klebsiella pneumoniae strains came from the same clone, which suggesting it might be an outbreak of nosocomial infection.