HIV感染者Th17和Treg细胞的变化及意义

Significance of change in Th17 and Treg cells in HIVinfected patients

摘要:

目的观察人免疫缺陷病毒（HIV）感染者辅助性T细胞（T help cell，Th）17和调节性T细胞（regulatory T cell, Treg）的数量、平衡状态变化及其意义。方法选取确诊的106例HIV感染者和30例健康体检者(健康对照组)，采集静脉血，以流式细胞术检测CD4+T细胞及Th17细胞与Treg细胞百分比，RTPCR检测HIV RNA病毒载量。结果HIV感染者CD4+T细胞为13～702个/μL，分为4组，即A组：≤200个/μL, 19例；B组：201~350个/μL ，32例；C组：351~500个/μL，26例；D组：＞500个/μL ，29例；上述各组均明显低于健康对照组（798~1 130个/μL）（P<0.05）。A、B、C、D组Th17细胞在CD4+T细胞中的百分比分别为(0.73±0.21)%、 (1.52±0.33)%、(2.92±0.46)% 、（3.74±0.58）%，均低于健康对照组的(4.90±1.43)％（P<0.05）；A、B、C、D 组及健康对照组Treg细胞在CD4+T细胞中的百分比分别为(9.78±2.15)%、(7.23±1.72)%、(5.69±1.67)%、(4.62±0.97)% 、(4.43±0.97)％，除D组与健康对照组之差异无统计学意义（P>0.05），其余组均高于健康对照组（P＜0.05）。HIV感染者病毒载量水平为(0～5.36)×107拷贝/mL，平均(5.36±1.21)×106拷贝/mL；A、B、C、D组的病毒载量分别为（6.24±1.36）×107拷贝/mL、（4.36±1.02) ×106拷贝/mL、（5.78±1.67）×104拷贝/mL、（3.54±1.25) ×103 拷贝/mL，各组间比较，差异有统计学意义（P<0.05），且与CD4+T细胞计数呈负相关。结论HIV感染导致Th17和CD4+T细胞下降、Th17和Treg细胞失衡，与HIV RNA载量负相关；与疾病进展密切相关。

关键词:

人免疫缺陷病毒;艾滋病;Th17细胞; Treg细胞; CD4+T细胞

Abstract:

ObjectiveTo evaluate the significance of change in T help cell 17(Th17) and regulatory T cell （Treg） in HIVinfected patients. Methods106 HIVinfected patients and 30 healthy volunteers were selected. Flow cytometry was used to detect the CD4+ T ,Th17 and Treg cells in peripheral blood. HIV RNA levels in serum were tested by RTPCR.ResultsCD4+ T cells were 13-702/uL, 19 patients were ≤200/uL (group A), 32 were 201-350/uL（ group B）, 26 were 351-500/uL（group C）, 29 were >500/uL（group D）; All were significantly lower than （798-1 130/uL） in healthy control group（P<0.05）. The percentage of Th17 cells in CD4+ T cells were (0.73±0.21)%,(1.52±0.33)%, (2.92±0.46)% , and (3.74±0.58）% in group A, B ,C and D, respectively, which were all lower than (4.90±1.43)％ of control group（P<0.05）; The percentage of Treg cells in CD4+ T cells were (9.78±2.15)%, (7.23±1.72)%, (5.69±1.67)% , and (4.62±0.97)% in group A, B,C and D,respectively, group A, B and C were higher than (4.43±0.97)％ of control group（P<0.05）, but group D had no significant difference compared with control group（P>0.05）. HIV RNA levels were (0-5.36)×107copies/mL in HIVinfected patients, the average levels of HIV RNA were (5.36±1.21)×106copies/mL ; HIV RNA levels in group A,B,C and D were（6.24±1.36）×107copies/mL，（4.36±1.02) ×106 copies/mL，（5.78±1.67）×104copies/mL，and （3.54±1.25) ×103copies/mL ,respectively，there was significant difference between each group(P<0.05), HIV RNA levels were negatively correlated with CD4 +T cells count. ConclusionHIV infection can result in the decrease of Th17 and CD4+T cells， imbalance of Th17/Treg cells， and negative correlation with HIV RNA levels.

Key words:

human immunodeficiency virus;acquired immunodeficiency syndrome; Th17 cell; Treg cell; CD4+T cell