儿童医院住院患儿铜绿假单胞菌的同源性

Research on homology of Pseudomonas aeruginosa isolated from hospitalized children

摘要:

目的了解某儿童专科医院临床分离的铜绿假单胞菌(PA)感染流行趋势，观察PA的科室分布特征、耐药性及基因型。方法收集该儿童专科医院住院患儿送检标本分离的56株PA进行耐药性分析，并应用脉冲场凝胶电泳(PFGE)分型技术对其进行基因分型。结果56株PA对氨苄西林耐药率为100.00%，对阿米卡星、环丙沙星耐药率为0.00%，对其他抗菌药物表现为不同的耐药率（1.79%～96.43%）；共被分为18个PFGE型别，其中A型26株（均分离于呼吸科），B型12株（11株分离于内分泌科）， C型3株，D～R型各1 株。不同科室分离的菌株数量不同，其中呼吸科分离最多，达27株（48.21%）；其次为内分泌科13株（23.21%）。结论该儿童医院呼吸科和内分泌科分别存在PA的2种不同克隆菌株流行。PFGE具有特异性高、重复性好、结果容易判断等优点，是目前细菌基因分型较为可靠的技术。

Abstract:

Objective To realize the molecular epidemiology and antimicrobial susceptibility patterns of Pseudomonas aeruginosa (PA)in a children's hospital.MethodsFiftysix  strains of PA isolated  from hospitalized children  were performed antimicrobial susceptibility test and pulsedfield gel electrophoresis( PFGE) genotyping.ResultsAll strains were resistant to ampicillin, resistant rate was 100.00%， and the resistant rate to amikacin and ciprofloxacin were both 0.00%, the resistant rates to the other antimicrobial agents  were between 1.79%-96.43%; isolates were  classified into 18 types by PFGE typing，26 of which were type A (all  were isolated from children in respiratory department )  , 12 were type B(11 strains from endocrinology department), 3 were type C , and 1 was type DR each , the isolated bacteria varied with different department, respiratory tract department had the most isolated strains (27 strains, 48.21%), the next was endocrinology department(13 strains, 23.21%).ConclusionTwo different major epidemic isolates of PA were isolated from  respiratory and endocrinology department in this hospital.  PFGE typing is a reliable method to characterize the prevalence isolates.