OXA23基因阳性耐亚胺培南鲍曼不动杆菌感染暴发调查研究 FREE

An infection outbreak of OXA23 carbapenemaseproducing imipenemresistant Acinetobacter baumannii FREE

摘要:

目的了解某院神经内科重症监护室 （ICU） 暴发流行耐亚胺培南鲍曼不动杆菌感染的基因型及耐药机制。方法以API20NE鉴定菌株，纸片扩散法测定分离菌株对常用抗菌药物的敏感性，聚合酶链反应 （PCR） 扩增3种酶基因 （OXA、IMP、VIM）,并对产物进行测序。结果患者痰标本分离的7株耐亚胺培南鲍曼不动杆菌均携带OXA23基因，未检出IMP、VIM基因。结论此次暴发流行中，耐碳青霉烯类药物的鲍曼不动杆菌携带的耐药基因为OXA23基因。

Abstract:

ObjectiveTo evaluate the gene types and drugresistance mechanism of imipenemresistant Acinetobacter baumannii (A. baumannii) causing infection outbreak in an intensive care unit (ICU) . MethodsA. baumannii isolates were identified by API20 NE, antimicrobial susceptibility were detected by disc agar diffusion methods. Three genes including OXA, IMP, and VIM were amplified by polymerase chain reaction (PCR), sequences were analysed using BLAST 2.0. ResultsSeven imipenemresistant A. baumannii isolates all carried OXA23 gene, IMP and VIM genes were not detected.ConclusionThe outbreak was mainly caused by A.baumannii which carried OXA23 gene.