脑膜炎奈瑟菌病原学及药物敏感性分析 FREE

Etiology and drug sensitivity of Neisseria meningitidis FREE

摘要:

目的了解某省各市、县疾病预防控制中心2007—2008年分离的8株脑膜炎奈瑟菌的病原学特征及药物敏感性。方法经培养及生化鉴定后，对菌株进行血清学及聚合酶链反应（PCR）鉴定分群，最后采用最低抑菌浓度（MIC）琼脂稀释法进行药敏试验。结果8株脑膜炎奈瑟菌中有5株C群、1株B群、1株Y群及1株不可分群菌株。所有菌株对青霉素、氨苄西林、米诺环素、头孢曲松、头孢噻肟、美罗培南、利福平敏感，对复方磺胺甲口恶唑则全部耐药，对环丙沙星和左氧氟沙星各有4株耐药，对氯霉素和阿奇霉素各有1株耐药。结论8株脑膜炎奈瑟菌的流行株中以C群为主；青霉素、头孢曲松、头孢噻肟、美罗培南等可作为治疗流行性脑脊髓膜炎的一线药物，脑膜炎奈瑟菌对喹诺酮类及大环内酯类抗生素的耐药现象需引起重视。

Abstract:

ObjectiveTo understand the pathogenic characteristics and drug sensitivity of 8 strains of Neisseria meningitidis isolated in Hunan province between 2007—2008.MethodsNeisseria meningitidis strains were identified and clustered by serology and PCR test, drug sensitivity test was carried by minimum inhibitory concentration (MIC) agar dilution method.ResultsAmong  8 strains of Neisseria meningitidis, 5 strains were in serogroup C, 1 in serogroup B, 1 in serogroup Y and 1 was unidentified. All 8 strains were sensitive to penicillin, ampicillin, minocycline, ceftriaxone, cefotaxime, meropenem, rifampicin, but resistant to sulfamethoxazole/trimethoprim,  4 strains were resistant to ciprofloxacin and levofloxacin respectively, and 1 strain was resistant to chloramphenicol and azithromycin respectively.ConclusionEight epidemic strains of Neisseria meningitidis are mainly in serogroup C; penicillin, ceftriaxone, cefotaxime and meropenem should be used as the firstline drugs for the treatment of epidemic cerebrospinal meningitis, the resistance to quinolones and macrolides should be paid attention.