抑制性寡脱氧核苷酸对肝损伤小鼠pSTAT4、pSTAT6和Tbet表达的影响及其意义 FREE

The effect of suppressive oligodeoxynucleotide on pSTAT4, pSTAT6 and Tbet expression of concanavalin Ainduced liver injury in micee FREE

摘要:

目的探讨抑制性寡脱氧核苷酸（suppressive oligodeoxynucleotide，Sup ODN）对刀豆蛋白A（ConA）诱导肝损伤小鼠兔抗鼠磷酸化信号转导和转录激动子4抗体 (pSTAT4) 、pSTAT6和Tbet表达的影响及其意义。方法Balb/C小鼠40只被随机分为4组，分别为生理盐水（NS，空白对照）组、环孢菌素A（CsA）组（阳性对照）、Con ODN组（对照ODN）、Sup ODN组（实验组）。ConA（20 mg/kg）尾静脉注射Balb/C小鼠造模，造模同时分别给予Sup ODN、Con ODN、CsA或 NS 0.3 mL腹腔注射，分别于干预后8 h和24 h摘取小鼠脾脏，提取蛋白检测pSTAT4、pSTAT6和Tbet的表达。结果无论是干预后8 h还是24 h，Sup ODN组pSTAT4及Tbet蛋白的表达水平与NS、Con ODN及CsA组相比，均显著下降（均P<0.01）；而pSTAT6蛋白表达水平，Sup ODN组与NS、Con ODN及CsA组差异均无显著性（均P>0.05）。结论Sup ODN可能通过抑制细胞内转录因子pSTAT4和Tbet的表达，抑制小鼠Th1型免疫应答，最终实现其对肝损伤的保护作用。

Abstract:

ObjectiveTo investigate the effect of suppressive oligodeoxynucleotide (Sup ODN) on pSTAT4, pSTAT6 and Tbet expression of concanavalin A induced liver injury in mice.MethodsForty Balb/C mice were randomly divided into 4 groups, normal saline group (NS, blank control group), cyclosporin A group (CsA group, positive control group) ,control oligodeoxynucleotide group (control ODN group), and Sup ODN group (trial group). Mice in each group were injected with ConA(20mg/kg) via the tail vein to make liver injury model. Sup ODN, Con ODN, CsA or NS was injected intraperitoneally at the same time of ConA injection. The expression of pSTAT4 ,pSTAT6 and Tbet in spleen at the 8th or 24th hour after intraperitoneal injection was examined by Westernblotting.ResultspSTAT4 and Tbet in Sup ODN group were significantly lower compared with NS，Con ODN or CsA group respectively ( all P< 0.01); pSTAT6 had no difference between Sup ODN group and other groups ( all P< 0.01).ConclusionSup ODN attained protective effect on ConAinduced mice liver injury by supressing expression of  pSTAT4 and Tbet.