痰涂片显微镜检查与培养结果的相关性分析 FREE

Correlation between sputum smear examination and bacterial culture results FREE

摘要:

目的分析痰涂片显微镜检查（镜检）与培养结果的相关性。方法痰标本在进行细菌培养之前行涂片革兰染色、镜检；对培养出来的细菌进行革兰染色、镜检，对二者镜下染色的性质和形态进行比对；二者一致者作为病原菌进行下一步的鉴定和药敏试验。结果786份合格标本共检出病原菌540株，阳性率68.70%，其中革兰阳性球菌185株，革兰阴性杆菌316株，真菌39株。痰涂片镜检与培养鉴定结果的符合率为72.01%（566/786），其诊断敏感性为77.78%,特异性为59.35%,阳性预测值为80.77%，阴性预测值为54.89%。结论痰涂片镜检结果与培养结果具有较高的符合率，痰涂片镜检可以提高痰标本病原菌的检出率。

关键词:

痰;痰涂片;显微镜检查;革兰染色;细菌培养;病原菌

Abstract:

The Second People’s Hospital of Liaocheng， Taishan Medical College， Linqing 252601， China )［Abstract］ObjectiveTo analyze the correlation  between  sputum smear examination (microscopy ) and bacterial culture results. MethodsSputum specimens were performed sputum smear and Gram’s staining before bacterial culture , and sputum cultured bacteria were also performed sputum smear and Gram’s staining, then observed under microscope. The staining characteristics and appearance of  two groups were evaluated; then further identification and antimicrobial susceptibility tests were carried out. ResultsFive hundred and forty pathogens were isolated from 786 qualified samples, the positive rate was 68.70%,  there were 185 strains of Grampositive cocci, 316 strains of Gramnegative bacilli and 39 strains of fungi. The coincidence between the results of two groups was  72.01%(566/786). The sensitivity of sputum smear was 77.78%, specificity 59.35%, positive predictive value 80.77%, negative predictive value 54.89%.ConclusionThere is a high coincidence rate between the results of sputum smear and bacterial culture, which can improve the detection rate of pathogenic bacteria.

Key words:

sputum; sputum smear; microscopic detection; Gram’s staining; bacterial culture; pathogen