MRSA分子流行病学研究 FREE

Molecular epidemiological study of methicillin resistant Staphylococcus aureus FREE

摘要:

目的 采用脉冲场凝胶电泳（PFGE）分型及多重聚合酶链反应（PCR）SCCmec分型技术对耐甲氧西林金黄色葡萄球菌（MRSA）进行基因分型，以了解医院感染MRSA的流行情况。方法 收集天津市南开医院2007年1月—2008年12月住院患者送检标本分离的MRSA 40株，进行耐药谱分析、PFGE分型和SCCmec分型。结果40株菌耐药谱高度相似，可被分为A—E 5个PFGE型，其中A型21株，B型8株，C型4株，D型6株，E型1株；SCCmec分型：Ⅰ型1株，Ⅲ型33株，Ⅳ型1株，V型5株。通过对医院内菌株发生的时间和空间分析，明确了感染的发生和传播情况，即在2007年1月—2008年12月存在MRSA散发感染。结论 PFGE可有效鉴定菌株间的亲缘关系，SCCmec分型对是否为医院感染菌株有提示作用，两种分型方法相结合可更好地鉴定流行菌株的特征，为医院感染的监控提供依据。

Abstract:

Objective To analyze the epidemiological characteristics of methicillin resistant Staphylococcus aureus (MRSA) in nosocomial infection with pulsed field gel electrophoresis (PFGE) and PCR SCCmec typing.Methods Forty strains  of MRSA  collected from Tianjin Nankai Hospital between January, 2007 and December, 2008  were performed  antimicrobial susceptibility analysis, PFGE and SCCmec genotyping.Results Antimicrobial susceptibility patterns of  40 strains of MRSA were highly similar,  isolates were classified into AE five types by PFGE typing，type A, B, C, D, E were 21, 8, 4, 6, 1 strains respectively； SCCmec typing showed that 1 strain was SCCmecⅠ,33 were SCCmecⅢ, 1 was SCCmecⅣ，and 5 were  SCCmecV. Sporadic infection of MRSA occurred in the hospital during the studied period. Conclusion Epidemiological analysis indicates that PFGE typing can identify the genetic relationship between isolates, SCCmec typing can  suggest whether  MRSA isolates are hospitalacquired .Combination of  two genotyping methods can show characteristics of epidemic strains, and provide reference for nosocomial infection surveillance.

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