尿液HIV1抗体检测及其临床意义 FREE

Detection and clinical significance of HIV1 antibody in FREE

摘要:

目的探讨尿液标本中人免疫缺陷病毒（HIV）1抗体检测的临床意义。方法应用酶联免疫吸附试验（ELISA）对59例经疾病预防控制中心（CDC）确诊的HIV感染者及30例健康体检者血液和尿液标本同时进行HIV1抗体检测；以确认结果为准，计算尿液HIV1抗体检测的敏感度、特异度。结果59例经CDC确诊的HIV感染者血清HIV1抗体全部阳性，相应尿液中HIV1抗体阳性53例，阴性6例；对照组30例的血清HIV1抗体全部阴性，相应尿液1例HIV1抗体阳性，经血清ELISA法及胶体硒法对该样本进行HIV1抗体检测均阴性。以确认结果为准，尿液HIV1抗体检测阳性敏感度为89.83%，特异度为96.67%。结论在采集血液标本不便的情况下，可通过检测尿液HIV1抗体对高危人群进行HIV感染筛查。

Abstract:

Detection and clinical significance  of HIV1 antibody in urineGUO Jingjing1, XU Dan2, WANG Min2, XIE Weiwei3, LIU Wenen1(1 Xiangya Hospital, Central South University, Changsha 410008, China; 2 Infectious Disease Hospital of Changsha,  Changsha 410011, China; 3 Department of Medical Laboratory of Xiangya School of Medicine,  Central South University, Changsha 410013, China)［Abstract］ObjectiveTo evaluate the clinical significance of detecting antiHIV1 antibody in urine samples.  MethodsAntiHIV1 antibody was  tested in serum and urine samples from 59 HIVinfected persons diagnosed by Provincial Center for Disease Control and Prevention and 30 healthy controls with  ELISA； Taking the results of the counterpart serum samples tested as standard, the sensitivity and specificity of  antiHIV1 antibody in urine samples were calculated.ResultsAmong 59 HIVinfected cases, antiHIV1 antibody positive results of serum test were observed in all cases, while 53 urine samples showed positive and 6 ones were negative. None of the serum samples showed antiHIV1 antibody positive in the control group, but 1 urine sample was positive, which was determined to be negative by serum enzymelinked immunosorbent assay and electroselenium assay. The sensitivity and specificity for the urine ELISA were 89.83% and 96.67% respectively. ConclusionThe results indicate that urine antiHIV1 antibody can be tested to screen HIV infection in high risk population, if serum samples are unavailable.