ICU多重耐药鲍曼不动杆菌医院感染的同源性分析 FREE

Homology analysis on ICU multidrugresistant Acinetobacter baumannii nosocomial infection FREE

摘要:

目的对某院重症监护室（ICU）流行的多重耐药鲍曼不动杆菌感染进行同源性分析。方法收集该ICU鲍曼不动杆菌感染流行期间住院患者标本中分离的9株以及环境中分离的24株鲍曼不动杆菌，采用基因外重复回文序列聚合酶链反应（REPPCR）技术对其进行基因分析，建立DNA指纹图谱；同时应用KB纸片扩散法进行药敏试验。结果9株临床分离的鲍曼不动杆菌共分为4个基因型，其中E1和E2型分别有4株和3株，E3和E4型各1株；环境样本分离的24株中，17株属E1型。药敏结果显示，临床分离株均为多重耐药株，环境分离株中19株为多重耐药株。结论此次ICU多重耐药鲍曼不动杆菌感染的流行主要是由E1和E2基因型在患者之间的相互传播所致，同时患者所处环境也被明显污染。

关键词:

鲍曼不动杆菌;重症监护室;医院感染;流行病学;多重耐药;抗药性，微生物;同源性

Abstract:

ObjectiveTo investigate an outbreak of nosocomial infection(NI) with multidrugresistant(MDR) Acinetobacter baumannii (A.baumannii) in an intensive care unit (ICU). MethodsNine strains isolated from patients and 24 strains isolated from   ICU environment during NI outbreak with MDR A.baumannii were typed by repetitive extragenic palindromicpolymerase chain reaction (repPCR) and antimicrobial susceptibility  was tested by KirbyBauer method. ResultsNine strains isolated from patients were    divided into 4 genotypes by repPCR,  type E1 and E2 had 4 and 3 strains respectively, both type E3 and E4 had 1 strain. 24 strains of  A .baumannii  were  isolated from the environmental samples, 17 of which belonged to type E1. Antimicrobial sensitivity test results showed that all clinical isolates and 19  environmental isolates  were  multidrugresistant strains.ConclusionThe MDR A.baumannii outbreak in ICU   was caused by transmission of E1 and E2 genotype A.baumannii among patients， and environment was also contaminated.

Key words:

Acinetobacter baumannii; intensive care units; nosocomial infection; epidemiology; multidrug resistance; drugresistance, microbial; homology