湖南省苗族健康人群外周血CD3+、CD4+、CD8+T淋巴细胞正常参考值研究

Normal reference values of peripheral blood CD3+, CD4+ and CD8+ T lymphocytes in healthy Miao ethnics in Hunan Province

摘要:

目的了解并建立湖南省苗族健康人群外周血CD3+、CD4+、CD8+T淋巴细胞正常参考值。方法应用FACSCalibur流式细胞仪检测10～19岁、20～29岁、30～39岁、40～49岁、50～59岁5个年龄组共360名健康苗族人的CD3+、CD4+、CD8+T淋巴细胞数值。结果10～59岁组苗族人群外周血CD3+、CD4+、CD8+T淋巴细胞绝对数和CD4+/CD8+比值分别为（1 426.71±462.92）个/μL、（787.54±258.36）个/μL、（589.86±242.91）个/μL和1.46±0.50；10～19岁年龄组的CD3+、CD8+T淋巴细胞计数均明显高于30岁以上年龄组（F分别为10.80、8.51，均P＜0.05），CD4+T淋巴细胞计数明显高于50～59岁组（F=7.03，P＜0.05）；不同性别间上述指标无差异（t分别为0.98、0.80、0.88、0.16，均P＞0.05）。结论建立不同民族的CD3+、CD4+、CD8+T淋巴细胞正常参考值有助于临床诊断。

Abstract:

ObjectiveTo understand and establish the normal reference values of peripheral blood CD3+, CD4+ and CD8+ T lymphocytes in healthy Miao ethnics in Hunan Province. MethodsThree hundred and sixty healthy  Miao ethnics were divided  into 5 age groups (10~19, 20~29, 30~39,40~49, 50~59), their  peripheral blood CD3+, CD4+ and CD8+ T lymphocytes were detected with FACSCalibur flowcytometry. ResultsThe mean absolute values of peripheral blood CD3+, CD4+, CD8+T lymphocytes and CD4+/CD8+ in healthy  Miao ethnics in 10～59 age groups were (1 426.71±462.92) cells/μL, (787.54±258.36) cells/μL, (589.86±242.91) cells/μL and 1.46±0.50 respectively, the mean absolute values of peripheral blood CD3+ and CD8+T lymphocytes in 10~19 age group were significantly higher than those in 30~59 age group (F=10.80,8.51, respectively, all P<0.05), CD4+T lymphocytes value was obviously higher than that in 50～59 age group (F=7.03, P＜0.05); There was on significant difference in above values between different genders (t=0.98, 0.80, 0.88, 0.16 respectively, all P＞0.05). ConclusionThe establishment of normal reference values of peripheral blood CD3+, CD4+ and CD8+T lymphocytes for healthy Miao ethnics will help to clinical diagnosis.